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hygromycin b  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hygromycin b
    Hygromycin B, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hygromycin/Hygromycin+B/pm42364981-222-16-18
    Average 99 stars, based on 1 article reviews
    hygromycin b - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Infection:

    Article Title: Fusion-positive rhabdomyosarcoma oncofusions share a common interactome.
    Article Snippet: .. After 18 hours, infected cells were pooled into a 15 cm culture dish and selected with 150 μg/ml hygromycin (Thermo Fisher Scientific). ..

    Transduction:

    Article Title: Inflammatory cytokines induce new cancer dependencies.
    Article Snippet: Collins K. Cheruiyot, Sarah Y. Kim, Juan Dubrot , Sarah K. Lane-Reticker, Alex Miranda, Ashwin V. Kammula , Jonathan J. Perera , Peter P. Du , Cun Lan Chuong, Rachel A. Fetterman, Nelson H. Knudsen , Aiping Jiang , Juliette S. M. T. Suermondt, Lomax F. Pass, Cong Fu, Meng-Ju Wu , Lei Shi , Seth Anderson , Audrey J. Muscato , Omar I. Avila , Ian C. Kohnle, Emily A. Kessler , Hans W. Pope, Sarah G. Noel , Kira E. Olander , Jooho Chung , Kayla J. Colvin, Nabeel Bardeesy, Kathleen B. Yates & Robert T. Manguso

    Cell Culture:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Article Title: Adenosine analogs for the treatment of disease
    Article Snippet: IB-MECA are obtained from Tocris (Bristol, UK) and N6-cyclopentyl adenosine (CPA) is obtained from Sigma-Alrich (Poole, UK). .. Cell culture media, G418 and hygromycin are obtained from Invitrogen (Paisley, UK). .. Fetal calf serum is obtained from Perbio Science (Cheshire, UK).

    Stable Transfection:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Expressing:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Binding Assay:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Flow Cytometry:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Negative Control:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Fluorescence:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    FACS:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Blocking Assay:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Suspension:

    Article Title: Antibody, nucleic acid, cell, and pharmaceutical
    Article Snippet: An artificial gene was synthesized using, as a base, a mouse LPA1 (NP_034466, SEQ ID NO: 112) that had been registered in NCBI gene bank, and introduced into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Inc.) to construct pEF-FRT-mLPA1. pEF-FRT-mLPA1 and pOG44 plasmid (Thermo Fisher Scientific, Inc.) were simultaneously introduced into Flp-In-CHO cell (Thermo Fisher Scientific, Inc.) using Lipofectamine 2000. .. The resulting cell was cultured in a culture medium containing hygromycin (Thermo Fisher Scientific, Inc.) to clone a hygromycin-resistant cell that stably expressed mouse LPA1 (hereinafter referred to as “mouse LPA1-stably expressing CHO cells”). (4) Evaluation for Binding Property of Serum Antibody to Human LPA1 and Mouse LPA1 by Flow Cytometry The human LPA1-stably expressing CHO cell, mouse LPA1-stably-expressing CHO cell, and a CHO-K1 cell or CHO-FlpIn cell (hereinafter referred to as “negative control cell”) were washed with a fluorescence-activated cell sorting (FACS) buffer (PBS containing 1% of fetal bovine serum (FBS)), to which Fc Block (Cytek Biosciences, Inc.) was added in an amount of 1/500 of the cell suspension to block the cells at 4° C. for 30 minutes. ..

    Selection:

    Article Title: A Novel Drug Candidate that Selectively Targets the Critical Androgen Receptor-ELK1 Growth Axis in Advanced and Drug-Resistant Prostate Cancer
    Article Snippet: The two types of recombinant cells were grown in DMEM supplemented with 5% FBS or 10% FBS, respectively, and 100 units/mL penicillin, 100 μg/mL streptomycin, 2mM L-glutamine mixture (Invitrogen). .. The selection antibiotics used in maintenance cultures of the two recombinant cell types were 100 μg/mL Hygromycin (Invitrogen) (to maintain GAL4-ELK1), 100 μg/mL Geneticin (Invitrogen) (to maintain Gal4-TATA-Luc) and 2 μg/mL Puromycin (Sigma-Aldrich) (to maintain AR) and 400 ug/mL Geneticin (Invitrogen) (to maintain ARE-TATA-Luc). ..

    Recombinant:

    Article Title: A Novel Drug Candidate that Selectively Targets the Critical Androgen Receptor-ELK1 Growth Axis in Advanced and Drug-Resistant Prostate Cancer
    Article Snippet: The two types of recombinant cells were grown in DMEM supplemented with 5% FBS or 10% FBS, respectively, and 100 units/mL penicillin, 100 μg/mL streptomycin, 2mM L-glutamine mixture (Invitrogen). .. The selection antibiotics used in maintenance cultures of the two recombinant cell types were 100 μg/mL Hygromycin (Invitrogen) (to maintain GAL4-ELK1), 100 μg/mL Geneticin (Invitrogen) (to maintain Gal4-TATA-Luc) and 2 μg/mL Puromycin (Sigma-Aldrich) (to maintain AR) and 400 ug/mL Geneticin (Invitrogen) (to maintain ARE-TATA-Luc). ..

    other:

    Article Title: Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.
    Article Snippet: All final plasmids were verified by full plasmid sequencing (Plasmidsaurus).



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